Determination of heavy metal concentrations in vegetables and agricultural soils is crucial because high levels of heavy metals could affect soil quality, crop production and safe consumption of crops. A field study was conducted to determine the heavy metal concentrations and their transfer from agricultural soils to different parts (leaf, stem, and root) of Brassica juncea (L.) Czern. In addition, potential health risks of contamination in the vegetables grown in the field were evaluated. Acid digestion method USEPA 3050B in combination with ICP-OES were used to analyze heavy metal (Al, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn) contents in both pre- and post-harvest soils and vegetable samples. Results showed that none of the heavy metals in soils had concentrations above the maximum safety limits based on the WHO, USEPA and CCME guidelines. Calculated metal transfer factor (MTF >1) showed B. juncea accumulated Cd, Co, Ni, Pb and Zn in leaves, stems and roots, but Cu and Mn, as well as Cr were only accumulated in stems and roots, respectively. There were variations in heavy metal contents between the different parts of B. juncea, but only Cd and Pb contents were above the maximum allowable limit recommended by FAO/WHO. PCA analysis was able to identify 4 major components corresponding to 38.38%, 28.98%, 14.39% and 10.67% of the total variance and PC1 was clearly associated to leaves of B. juncea. Based on the MTF values, only Cd was found to have a value of HRI >1 compared to the other heavy metals, implying potential health risk associated with long-term ingestion of the vegetable.